Analisis asam retinoat dalam sediaan krim anti jerawat di Kota Jambi menggunakan spektrofotometri UV-VIS

Abstract

Retinoic acid is widely used as an additive in cream bleach, retinoic acid is a list of ingredients prohibited in cosmetics under the rules of the POM 2011. Many cases of the use of retinoic acid found in cosmetics that have a negative impact on health. This study aims to determine the levels of retinoic acid contained in the anti-acne cream on the market in Jambi.  Used thin layer chomatography and UV-VIS Spectrophotometry. Results of identification by TLC shows Rf 0.58 whereas retinoic acid comparison of samples 1,2 and 6 obtained Rf 0.59, 0.58, 0.58 and 3.4 samples and 5 are not formed stain (black spot). Results of the determination of the maximum wavelength of retinoic acid with UV-Vis spectrophotometer gained 348.20 nm. The results of manufacture calibration curve, obtained the regression equation y = 1,25835x - 0.09127 with coefficient correlation (r) of 0.99990. Acid assay results retinoic positive samples, sample 1 = 0.00687%, sample 2 = 0.0118%, and the sample 6 = 0.00412%. Conclusion is From 6 samples just three samples containing retinoic acid, the levels allowed in the preparation of anti-acne cream that is below 0.1%.